Image Processing - What happens after scanning?
- When the scan is complete, the file is automatically saved in .txrm format.
file is opened into the image reconstructor software, where the raw
images are "put together" into 3D form. The file is saved as an image
stack in a variety of possible output formats including: dicom, bin, or
reconstructed file is opened in an
appropriate image processing software for the desired purpose. Our
workstation has the following softwares which are accessible to all
users: Amira 5.4.2, Avizo, ImageJ, Matlab. Once the data set is
uploaded, both 2D image slices and a 3D rendering is automatically
attainable with a viewer. Quantitative results may also be found
through image thresholding and segmentation techniques, and allows for
acquiring differences in intensity (grayscale changes) or mineral
density changes. Volumes of specific features can also be determined,
for example the volume of a specific mineralized tissue (enamel,
dentin, bone), volume of vasculature networks, volume of
osteocyte/lacunae, or even soft tissue. *Note- for imaging soft tissue,
an appropriate staining/contrast agent must be used.