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The MicroXCT-200 is a 3D tomographic x-ray transmission microscope. This bench top unit has a high spatial resolution (0.5 μm) and can sample non-living tissues to see critical internal structure, features, and defects. The instrument can scan both mineralized and soft tissues such as bone osteocytes, Sharpey’s fiber inserts, vasculature, peritubular dentin, PDL, and embryonic tissues. The MicroXCT-200 will complement other high resolution imaging techniques like atomic force microscopy and will foster new opportunities through interdepartmental and campus wide collaborations. This facility is also open to outside users within the UC systems, other universities, and industries. Like a CAT-scan (computer aided tomography) at the micron scale, this instrument is economical, versatile, and an easy-to-use tool for a wide range of applications. The approximate operable energy range of this system is ~30kV-90kV, and the available magnifications are: 2X, 4X, 10X, 20X, and 40X. The stage can rise to a maximum height of ~40 mm.